Fluorescence Detector


World-class sensitivity meets diverse needs

Prominence RF-20A/RF-20Axs

The excellent basic performance of the Prominence series is further enhanced by the RF-20A/RF-20Axs fluorescence detectors, which offer world-class sensitivity, excellent ease of maintenance, and validation support functions. They support a wide range of applications from conventional analysis to high-performance analysis.

  • Achieves world-class sensitivity
  • Cell temperature control enhances reproducibility
  • Support for ultra-fast analysis
  • Easy maintenance
  • Specifications
Achieves world-class sensitivity

Utilizing a newly designed optical system, the RF-20A and RF-20Axs offer world-class levels of sensitivity. A water Raman S/N ratio of at least 2000 for the RF-20Axs and 1200 for the RF-20A makes these detectors powerful tools for analyses demanding the detection of trace-level components. The diagram at right shows an example of the ultra-high-sensitivity analysis of anthracene. A S/N ratio of 21.5 was achieved for an injection of 10.48 fg anthracene (RF-20Axs). This is equivalent to approx. 1.5 fg limit of detection (S/N ratio = 3), which is excellent.

Cell temperature control enhances reproducibility (RF-20Axs)

The fluorescence intensity drops as the temperature rises. A fluctuation of about 1°C near room temperature may result in approximately 5% intensity fluctuations for some compounds. To prevent this, the RF-20Axs features a temperature-controlled cell with a cooling function. It maintains a constant detector cell temperature, even if the room temperature fluctuates significantly, to ensure superb reproducibility with no drop in sensitivity. As shown below, without temperature control, the peak area value dropped approximately 17% due to the increase in cell temperature when the room temperature changed from 25 °C to 30 °C. Good accuracy could not be obtained, with reproducibility of 6.3% RSD (n=6). With its temperature-controlled cell, the RF-20Axs is not affected by such fluctuations in room temperature.

Cell temperature control enhances reproducibility (RF-20Axs)

Effect of Temperature-Controlled Cell (Acridine)

Support for ultra-fast analysis

Fast response is required to follow the sharp peaks obtained by ultra-fast analysis. The 10 ms response of the RF-20A and RF-20Axs permits ultra-fast analysis with no loss of separation. In addition, the highly sensitive simultaneous analysis of multiple components requires detection at the optimal wavelengths. The RF-20A and RF-20Axs permit ultra-fast, high-sensitivity multi-component analysis using wavelength switching via a time program.

Support for ultra-fast analysis

Ultra-Fast Simultaneous Analysis of 15 Polycyclic Aromatics

A: Excitation wavelength (Ex): 270 nm; Emission wavelength (Em): 330 nm, B: Ex: 250nm Em: 370nm, C: Ex: 330 nm Em: 430 nm, D: Ex: 290 nm Em: 390nm, E: Ex: 290 nm Em: 430nm, F: Ex: 370 nm Em: 460 nm, G: Ex: 270 nm Em: 330 nm

Easy maintenance

Maintenance from front panel

The Xenon lamp and flow cell can be replaced at the front panel. No positional adjustment is required when replacing the Xenon lamp, and no tools are required to replace the flow cell. The standard flow cell or semimicro flow cell can be rapidly replaced.

Maintenance from front panel

Maintenance from front panel

Long-life lamp reduces running costs

The Xenon lamp life has been extended to 2000 hours, four times longer than previous Shimadzu lamps. This significantly reduces running costs and downtime due to maintenance.

RF-20A and RF-20Axs Fluorescence Detector Specifications

  RF-20A RF-20Axs
Light Source Xenon lamp Xenon lamp, Low-pressure mercury lamp (to check wavelength accuracy)
Wavelength Range 0, 200 nm to 650 nm
(Dual wavelength simultaneous monitoring possible)
0, 200 nm to 750 nm
(Dual wavelength simultaneous monitoring possible)
Spectral Bandwidth 20nm
Wavelength Accuracy ±2 nm
Wavelength Reproducibility ±0.2 nm
S/N Water Raman peak S/N 1200 min. Water Raman peak S/N 2000min.
Cell (capacity, pressure resistance, material) 12 µL; 2 MPa (approx. 20 kgf/cm2); SUS316L, PTFE (fluororesin), quartz
Cell Temperature Input Range 4°C
to 40 °C, 1 °C step
Cell Temperature Control Range (Room temperature - 10°C) to 40°C

What is a fluorescence detector?

A fluorescence detector excites the sample with excitation light and breaks up the emitted fluorescence light with a fluorescence monochromator. It extracts the required fluorescence wavelengths and measures the intensity with a photomultiplier.

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